EVALUATION AND COMPARISON OF TOTAL ANTIOXIDANT CAPACITY OF SALIVA IN PATIENTS WITH AND WITHOUT CHRONIC PERIODONTITIS PATIENT
Background: Saliva despite being rich in ions, proteins, and antibodies also contains antioxidants: ascorbic acid, uric acid, albumin, and enzymes, which play an important role against oxidative stresses (OS) that causes oral cavity diseases like periodontitis. The aim of study is to analyze and compare the total antioxidant capacity of saliva between chronic periodontitis patients (at baseline and after therapy) and healthy non-chronic periodontitis patients (control group) in addition to comparing periodontal measurements such as gingival index, pocket depth and clinical attachment loss among chronic periodontitis patients at baseline and after one month from treatment.
Methods: The methodology of this study is a clinical quantitative study, which consists of A total of 53 patients who visited Duhok dental polyclinic/ Kurdistan region of Iraq from April 2021 to November 2021 were enrolled in this study. Among them, 37 patients suffered from chronic periodontitis, while 16 individuals were healthy and regarded as control group, age group was between 30 and 55 years old, out of 52 patients, 23 were female and 30 were male. Unstimulated saliva samples were collected from patients at baseline and one month after therapy (non-surgical treatment) and from control group. All samples were analyzed using total Antioxidant Capacity Assay Kit (Sigma-Aldrich, USA) by EPOCH2 plate reader (BioTek, USA).
Results: The means of periodontal parameters (Gingival Index, Periodontal Pocket Depth, Clinical Attachment Loss) in chronic periodontitis patients were significantly decreased after non-surgical treatment, while the Total Antioxidant Capacity (TAC) of saliva was significantly increased in chronic periodontitis patients after one month of treatment but still significantly lowered when compared with the control group.
Conclusions: The Total Antioxidant Capacity (TAC) of saliva decreased in chronic periodontitis patients as the result of oxidative stress generated by bacterial pathogens but elevated by non-surgical treatment and good oral hygiene home care.
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