VITAMIN D RECEPTOR GENE APAI AND TAQI POLYMORPHISM IN PATIENTS WITH TYPE II DIABETES MELLITUS USING PCR-RFLP METHOD IN KURDISTAN REGION
Background: Type 2 diabetes mellitus that characterized by insulin resistance and it is a risk of many diseases the impact of genetic factors on diabetes is well documented. Vitamin D receptor (VDR) gene polymorphisms have been linked to T2DM. In this study, we analyzed the relation between TaqI and ApaI VDR gene polymorphisms and T2DM subjects by using the PCR-RFLP method in Kurdish patients.
Material and Methods: Forty patients with T2DM and 30 uninflected individuals were included in this study. Genomic DNA was amplified using PCR and VDR gene was analyzed by the PCR-RFLP method. The ApaI product G allele yielded fragments of 528 and 217 bp; T allele, 745 bp, and TaqI T allele yielded fragments of 494 and 251 bp; C allele, 293, 251, and 201 bp.
Results: Forty patients with T2DM and 30 uninflected individuals have been studied, both patients and the control group were age-matched, (43.59%) of patients have had a family history of type2 Diabetes. The allele and genotype frequencies of the VDR TaqI (g.60058 T>C) gene and VDR ApaI (g.59979 G>T) gene polymorphisms were investigated. In VDR TaqI (T>C) gene polymorphism genotypes are expressed as TT in normal wild-type homozygote, TC in the heterozygote, and CC in the homozygote mutant polymorphic genotype. There were statistically significant differences between patients withT2DM and controls regarding the distribution of TaqI genotypes and alleles (p< 0.0001) and no significant difference regarding ApaI alleles (P=0.5532).
Conclusion: Current study findings demonstrated no associations between ApaI polymorphism and Kurdish T2DM patients and only associations between VDR TaqI gene polymorphism, it can be assumed that VDR and its exon 9 polymorphism are crucial in the pathogenesis of T2DM.
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